WARNING: Internet Explorer does not support modern web standards. This site may not function correctly on this browser and is best viewed on Chrome, Firefox or Edge browsers. Learn More.

Sequential in-office vitreous aspirates demonstrate vitreous matrix metalloproteinase 9 levels correlate with the amount of subretinal fluid in eyes with wet age-related macular degeneration



To evaluate levels of 37 native pathway proteins of the vitreous proteome from a subset of wet age-related macular degeneration (AMD) patients with and without subretinal fluid (SRF).


A total of 62 consecutive samples were aspirated from 12 patients with AMD, six who had SRF at baseline, and six who did not have SRF at any point during the study. Vitreous levels of the 37 native pathway proteins were analyzed in these patients using reverse phase protein microarray technology. At each visit, at which the 62 samples were taken, SRF and central retinal thickness were measured. These values were then compared to the relative intensity level of the 37 proteins screened.


In the subset of AMD patients with SRF, the average matrix metalloproteinase 9 (MMP-9), interleukin (IL)-12, Abelson murine leukemia viral oncogene homolog 1 (cABL) Thr735, heme oxygenase-1, Musashi, platelet-derived growth factor receptor beta Tyr751 (PDGFRβ), IL-8, and BCL-2 associated death promoter (BAD) Ser112 levels in the vitreous were found to be significantly different with a 21%-82% increase in expression compared to those without SRF (p<0.0001). Within the SRF group, there was a positive correlation between the vitreous MMP-9 levels and the SRF level. MMP-9 levels in the vitreous proteome varied with the level of SRF but not retinal edema. Compared to patients without SRF, the patients with initial SRF had persistent or progressive disease.


This is the first prospective case series sequentially monitoring the vitreous proteome in patients with wet AMD. The results suggest that MMP-9 is a proteomic biomarker of SRF accumulation, separate from macular edema.

Are You Suffering From Flashes & Floaters?
Schedule a Retina Eye Exam Today!
Patient Portal Login

Our Locations


6325 Woodside Court
Suite 125
Columbia, Maryland 21046

(410) 919-4455
Fax: (410) 339-7326
Mon-Fri: 8:30am to 5:00pm
On Call 24/7 (410) 919-4455

Owings Mills

21 Crossroads Drive
Suite 425
Owings Mills, MD 21117

(410) 337-4500
Fax: (410) 339-7326
Mon-Fri: 8am-4:30pm
On Call 24/7 (410) 337-4500


5420 Olney Laytonsville Road
Olney, MD 20832

(410) 919-4455
Fax: (410) 339-7326
Mon-Fri: 8am-4:30pm
On Call 24/7 (410) 337-4500