WARNING: Internet Explorer does not support modern web standards. This site may not function correctly on this browser and is best viewed on Chrome, Firefox or Edge browsers. Learn More.

A prospective study of in-office diagnostic vitreous sampling in patients with vitreoretinal pathology



To evaluate the safety and efficacy of in-office vitreous sampling (50-100 microL).


A prospective, consecutive case series of 578 diagnostic vitreous sample attempts performed between February 23, 2007 and September 31, 2008. A standardized technique of vitreous sampling followed by intravitreal injection of therapeutic medication was performed. All subjects were followed for adverse events after sampling/injection.


Out of a total of 578 vitreous sampling attempts, a total of 550 positive diagnostic vitreous samples were collected from 157 eyes of 144 patients. There were a total of 28 “dry” samples in 19 subjects (13 females and 6 males). The rate of dry sample was 4.8% (28 of 578). Complications included transient vitreous hemorrhage (n = 3), transient intraocular inflammation (n = 3), localized, chronic retinal detachment (n = 1). None of the complications resulted in permanent visual sequelae. These complications were no different than those expected from intravitreal injection alone.


This prospective study has shown that in-office vitreous sampling of 50 microL to 100 microL is a safe and reproducible procedure. Larger randomized studies will be helpful to further assess the safety and long-term effects of in-office diagnostic vitreous sampling.

Read more about this research – http://www.ncbi.nlm.nih.gov/pubmed/19373124

Are You Suffering From Flashes & Floaters?
Schedule a Retina Eye Exam Today!
Patient Portal Login

Our Locations


6325 Woodside Court
Suite 125
Columbia, Maryland 21046

(410) 919-4455
Fax: (410) 339-7326
Mon-Fri: 8:30am to 5:00pm
On Call 24/7 (410) 919-4455

Owings Mills

21 Crossroads Drive
Suite 425
Owings Mills, MD 21117

(410) 337-4500
Fax: (410) 339-7326
Mon-Fri: 8am-4:30pm
On Call 24/7 (410) 337-4500


5420 Olney Laytonsville Road
Olney, MD 20832

(410) 919-4455
Fax: (410) 339-7326
Mon-Fri: 8am-4:30pm
On Call 24/7 (410) 337-4500